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Gene amiE from Helicobacter pylori 26695: acylamide amidohydrolase
Member of NCBI Protein Clusters PRK13286(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 900109
UniProtKB accession
RefSeq Protein accession NP_207092.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: amiE
  • locus tag: HP0294
Organism Helicobacter pylori 26695 (NCBI TaxID: 85962)
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Source Predicted function(s)
NCBI Protein Cluster Prediction acylamide amidohydrolase
Prof. Vitkup / Confidence:0.823 (References: 19935659, 16507154)
Berkeley Phylogenomics Group Nitrilase. / (References: 19435885)
Berkeley Phylogenomics Group GO:0047558 3-cyanoalanine hydratase activity / (References: 19435885)
Berkeley Phylogenomics Group GO:0018822 nitrile hydratase activity / (References: 19435885)
Berkeley Phylogenomics Group Amidase. / (References: 19435885)
Berkeley Phylogenomics Group GO:0047427 cyanoalanine nitrilase activity / (References: 19435885)
Berkeley Phylogenomics Group GO:0047710 bis(5'-adenosyl)-triphosphatase activity / (References: 19435885)
Berkeley Phylogenomics Group GO:0050126 N-carbamoylputrescine amidase activity / (References: 19435885)
Berkeley Phylogenomics Group GO:0080109 indole-3-acetonitrile nitrile hydratase activity / (References: 19435885)
Berkeley Phylogenomics Group GO:0080061 indole-3-acetonitrile nitrilase activity / (References: 19435885)
Source References Functional description
[PMID: 9364923]
[PMID: 11359566]
Aliphatic amidase (source organism: Helicobacter pylori (Campylobacter pylori).; strain specific ID: 85P)
Functional Status greenGreen (experimental evidence, uncurated)
EC number
GO terms
  • MF: GO:0004040 : amidase activity : IEA
  • MF: GO:0016787 : hydrolase activity : IEA
  • BP: GO:0006807 : nitrogen compound metabolic process : IEA
  • MF: GO:0016810 : hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds : IEA
Domain Structure from CDD
  • COG0388: Predicted amidohydrolase [General function prediction only]
  • aliphatic_amidase: aliphatic amidases (class 2 nitrilases). Aliphatic amidases catalyze the hydrolysis of short-chain aliphatic amides to f.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
amiE (6)
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
HP0292hypothetical protein
HP0293para-aminobenzoate synthetase (pabB)
Metabolic Annotation from Palsson Lab

Metabolic Reaction: AMID2

h2o[c] + pad[c] -> nh4[c] + pac[c]

Metabolic Reaction: AMID4

ad[c] + h2o[c] -> ac[c] + nh4[c]

Metabolic Reaction: AMID5

aa[c] + h2o[c] -> acryl[c] + nh4[c]

Source: Ported to COMBREX by Segre's Lab