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Gene dnaK from Escherichia coli str. K-12 substr. MG1655: chaperone Hsp70, co-chaperone with DnaJ
Member of NCBI Protein Clusters PRK00290(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 944750
UniProtKB accession
RefSeq Protein accession NP_414555.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: dnaK
  • locus tag: b0014
  • aliases: ECK0014, JW0013, groPAB, groPC, groPF, grpC, grpF, seg
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
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Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction molecular chaperone DnaK
Source References Functional description
GSDB
[PMID: 1396676]
[PMID: 9298646]
[PMID: 1592823]
[PMID: 2674651]
[PMID: 7783627]
[PMID: 1835085]
[PMID: 8206983]
[PMID: 16079137]
[PMID: 18723842]
[PMID: 8658133]
[PMID: 9609686]
[PMID: 10742174]
Chaperone protein dnaK (source organism: Escherichia coli (strain K12).; strain specific ID: )
Functional Status greenGreen (experimental evidence, uncurated)
GO terms
  • BP: GO:0006260 : DNA replication : IEA
  • MF: GO:0005524 : ATP binding : IEA
  • CC: GO:0005737 : cytoplasm : IDA
  • BP: GO:0006457 : protein folding : IEA
  • CC: GO:0005886 : plasma membrane : IEA
  • CC: GO:0016020 : membrane : IDA
  • MF: GO:0008270 : zinc ion binding : IDA
  • CC: GO:0016020 : membrane : IEA
  • CC: GO:0019898 : extrinsic to membrane : IEA
  • MF: GO:0000166 : nucleotide binding : IEA
  • CC: GO:0005737 : cytoplasm : IEA
  • MF: GO:0051082 : unfolded protein binding : IEA
  • BP: GO:0006950 : response to stress : IEA
  • BP: GO:0009408 : response to heat : IEP
  • MF: GO:0005515 : protein binding : IPI
Domain Structure from CDD
  • dnaK: molecular chaperone DnaK; Provisional

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
dnaK (105) , NT01EI_0672 , Tola_2257 , VCD_003472
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see www.java.com
 gene neighbourhoods
phylogenetic profile
dnaJchaperone Hsp40, co-chaperone with DnaK
 
 
Phenotype Description Expression
Class
Reference(s)
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(not essential)
PMID: 20029369
(not essential)
Ciprofloxacin sensitivity Knockout confers increased sensitivity to ciprofloxacin Knock out
PMID: 20065048
Colistin sensitivity Knockout confers increased sensitivity to colistin Knock out
PMID: 20065048
Erythromycin sensitivity Knockout confers increased sensitivity to erythromycin Knock out
PMID: 20065048
Fusidic Acid sensitivity Knockout confers increased sensitivity to fusidic Knock out
PMID: 20065048
Rifampicin sensitivity Knockout confers increased sensitivity to rifampicin Knock out
PMID: 20065048
Spectinomycin sensitivity Knockout confers increased sensitivity to spectinomycin Knock out
PMID: 20065048
Sulfamethoxazole sensitivity Knockout confers increased sensitivity to sulfamethoxazole Knock out
PMID: 20065048
Tetracycline sensitivity Knockout confers increased sensitivity to tetracycline Knock out
PMID: 20065048
Triclosan sensitivity Knockout confers increased sensitivity to triclosan Knock out
PMID: 20065048
Vancomycin sensitivity Knockout confers increased sensitivity to vancomycin Knock out
PMID: 20065048