Combrex Enter Keyword, gene symbol, or identifier (more options; Help )
Enter species name
Gene functional status (select)
Phenotype association (select)[View list of phenotypes]
Check to restrict search to:
Genes in Category A-C and Emerging/Re-Emerging pathogens
Genes in curated Protein Clusters
Genes with predictions submitted to COMBREX
Genes with solved structures in PDB
Genes with Pfam domains shared with human proteins
Genes with products purified by PSI
Genes cloned by PSI
close options


Gene rplY from Escherichia coli str. K-12 substr. MG1655: 50S ribosomal subunit protein L25
Member of NCBI Protein Clusters PRK05943(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 945618
UniProtKB accession
RefSeq Protein accession NP_416690.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: rplY
  • locus tag: b2185
  • aliases: ECK2179, JW2173
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
Initiate the grant application process for experimentally validating this gene (Important notice about COMBREX grants.)
Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction 50S ribosomal protein L25
Source References Functional description
[PMID: 1093874]
[PMID: 1100506]
[PMID: 9298646]
[PMID: 9868784]
[PMID: 3298242]
[PMID: 8925931]
[PMID: 10094780]
[PMID: 9799245]
[PMID: 10562563]
[PMID: 10696113]
[PMID: 12809609]
[PMID: 16272117]
50S ribosomal protein L25 (source organism: Escherichia coli (strain K12).; strain specific ID: MRE-600)
Functional Status greenGreen (experimental evidence, uncurated)
GO terms
  • MF: GO:0008097 : 5S rRNA binding : IEA
  • CC: GO:0030529 : ribonucleoprotein complex : IEA
  • CC: GO:0005622 : intracellular : IEA
  • BP: GO:0006412 : translation : IEA
  • MF: GO:0019843 : rRNA binding : IEA
  • MF: GO:0005515 : protein binding : IPI
  • MF: GO:0003723 : RNA binding : IEA
  • MF: GO:0003735 : structural constituent of ribosome : IEA
  • CC: GO:0005840 : ribosome : IEA
Domain Structure from CDD
  • Ribosomal_L25_TL5_CTC: Ribosomal_L25_TL5_CTC: Ribosomal L25/TL5/CTC N-terminal 5S rRNA binding domain. L25 is a single-domain protein, homologo.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
CKO_00591 , ECH74115_3323 , EcolC_1462 , ECP_2226 , ECs3077 , ECSE_2454 , ESA_01051 , KPK_1543 , rplY (38) , SARI_00663 , SeD_A2575 , SPAB_00776 , SSPA0587
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 phylogenetic profile
lntapolipoprotein N-acyltransferase
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(not essential)
PMID: 20029369
(not essential)