Combrex Enter Keyword, gene symbol, or identifier (more options; Help )
Enter species name
Gene functional status (select)
Phenotype association (select)[View list of phenotypes]
Check to restrict search to:
Genes in Category A-C and Emerging/Re-Emerging pathogens
Genes in curated Protein Clusters
Genes with predictions submitted to COMBREX
P
Genes with solved structures in PDB
S
Genes with Pfam domains shared with human proteins
H
Genes with products purified by PSI
U
Genes cloned by PSI
C
close options

 

Gene aspS from Escherichia coli str. K-12 substr. MG1655: aspartyl-tRNA synthetase
Member of NCBI Protein Clusters PRK00476(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 946385
UniProtKB accession
RefSeq Protein accession NP_416380.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: aspS
  • locus tag: b1866
  • aliases: ECK1867, JW1855, tls
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 1C0A 1EQR 1IL2
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG10097
Initiate the grant application process for experimentally validating this gene (Important notice about COMBREX grants.)
Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction aspartyl-tRNA synthetase
Source References Functional description
GSDB
[PMID: 10562565]
[PMID: 10873442]
Aspartyl-tRNA synthetase (source organism: Escherichia coli (strain K12).; strain specific ID: )
Functional Status greenGreen (experimental evidence, uncurated)
EC number
GO terms
  • BP: GO:0006422 : aspartyl-tRNA aminoacylation : IEA
  • MF: GO:0005524 : ATP binding : IEA
  • CC: GO:0005829 : cytosol : IDA
  • MF: GO:0016874 : ligase activity : IEA
  • MF: GO:0004815 : aspartate-tRNA ligase activity : IEA
  • MF: GO:0003676 : nucleic acid binding : IEA
  • BP: GO:0006418 : tRNA aminoacylation for protein translation : IEA
  • MF: GO:0000166 : nucleotide binding : IEA
  • MF: GO:0004815 : aspartate-tRNA ligase activity : IDA
  • CC: GO:0005737 : cytoplasm : IEA
  • MF: GO:0004812 : aminoacyl-tRNA ligase activity : IEA
  • BP: GO:0006412 : translation : IEA
  • BP: GO:0006422 : aspartyl-tRNA aminoacylation : IDA
Domain Structure from CDD
  • aspS: aspartyl-tRNA synthetase; Validated
  • AspRS_core: Asp tRNA synthetase (aspRS) class II core domain. Class II assignment is based upon its structure and the presence of th.... (More)
  • GAD: GAD domain. This domain is found in some members of the GatB and aspartyl tRNA synthetases.
  • EcAspRS_like_N: EcAspRS_like_N: N-terminal, anticodon recognition domain of the type found in Escherichia coli aspartyl-tRNA synthetase .... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
aspS (68)
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see www.java.com
 gene neighbourhoods
phylogenetic profile
nudBdihydroneopterin triphosphate pyrophosphatase
 
 
rplS50S ribosomal subunit protein L19 
 
rluApseudouridine synthase for 23S rRNA (position 746) 
 
rpmI50S ribosomal subunit protein L35 
 
frrribosome recycling factor 
 
truCtRNA U65 pseudouridine synthase 
 
rpsR30S ribosomal subunit protein S18 
 
rluC23S rRNA pseudouridine synthase; specific to U955, 
 
obgEGTPase involved in cell partioning and DNA repair 
 
rplL50S ribosomal subunit protein L7/L12 
 
rpsF30S ribosomal subunit protein S6 
 
mnmAtRNA (5-methylaminomethyl-2-thiouridylate)-methylt 
 
fmt10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N 
 
rpmG50S ribosomal subunit protein L33 
 
ftsHprotease, ATP-dependent zinc-metallo 
 
rpsP30S ribosomal subunit protein S16 
 
pthpeptidyl-tRNA hydrolase 
 
tufBprotein chain elongation factor EF-Tu (duplicate o 
 
ssbsingle-stranded DNA-binding protein 
 
rncRNase III 
 
rpmA50S ribosomal subunit protein L27 
 
tsfprotein chain elongation factor EF-Ts 
 
lepAback-translocating Elongation Factor EF4, GTPase 
 
arnAfused UDP-L-Ara4N formyltransferase/UDP-GlcA C-4'- 
 
acpPacyl carrier protein (ACP) 
 
rluD23S rRNA pseudouridine synthase; specific to U1911 
 
yidCmembrane protein insertase 
 
rplQ50S ribosomal subunit protein L17 
 
tufAprotein chain elongation factor EF-Tu (duplicate o 
 
rplI50S ribosomal subunit protein L9 
 
prfApeptide chain release factor RF-1 
 
Phenotype Description Expression
Class
Reference(s)
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(candidate essential)