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Gene gltX from Escherichia coli str. K-12 substr. MG1655: glutamyl-tRNA synthetase
Member of NCBI Protein Clusters PRK01406(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 946906
UniProtKB accession
RefSeq Protein accession NP_416899.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: gltX
  • locus tag: b2400
  • aliases: ECK2394, JW2395
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB:
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG10407
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Source Predicted function(s)
NCBI Protein Cluster Prediction glutamyl-tRNA synthetase
Source References Functional description
[PMID: 3015933]
[PMID: 8218204]
[PMID: 6280993]
[PMID: 6986385]
[PMID: 7797500]
[PMID: 14764088]
Glutamyl-tRNA synthetase (source organism: Escherichia coli (strain K12).; strain specific ID: )
Functional Status greenGreen (experimental evidence, uncurated)
EC number
GO terms
  • MF: GO:0046872 : metal ion binding : IEA
  • MF: GO:0005524 : ATP binding : IEA
  • MF: GO:0016874 : ligase activity : IEA
  • MF: GO:0008270 : zinc ion binding : IEA
  • MF: GO:0004818 : glutamate-tRNA ligase activity : IDA
  • BP: GO:0006418 : tRNA aminoacylation for protein translation : IEA
  • BP: GO:0006424 : glutamyl-tRNA aminoacylation : IEA
  • MF: GO:0008270 : zinc ion binding : IDA
  • BP: GO:0006424 : glutamyl-tRNA aminoacylation : IMP
  • MF: GO:0000166 : nucleotide binding : IEA
  • CC: GO:0005737 : cytoplasm : IEA
  • MF: GO:0004812 : aminoacyl-tRNA ligase activity : IEA
  • BP: GO:0006412 : translation : IEA
  • BP: GO:0006779 : porphyrin biosynthetic process : NAS
  • MF: GO:0004818 : glutamate-tRNA ligase activity : IEA
  • MF: GO:0005515 : protein binding : IPI
Domain Structure from CDD
  • gltX: glutamyl-tRNA synthetase; Reviewed
  • GluRS_core: catalytic core domain of descriminating glutamyl-tRNA synthetase. Descriminating Glutamyl-tRNA synthetase (GluRS) cataly.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
gltX (68) , NT01EI_1258
Metabolic Annotation from Palsson Lab

Metabolic Reaction: GLUTRS

Glutamyl-tRNA synthetase
atp[c] + glu-DASH-L[c] + trnaglu[c] -> amp[c] + glutrna[c] + ppi[c]
Cofactor and Prosthetic Group Biosynthesis

Source: Ported to COMBREX by Segre's Lab
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(candidate essential)