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Gene ispB from Escherichia coli str. K-12 substr. MG1655: octaprenyl diphosphate synthase
Member of NCBI Protein Clusters PRK10888(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 947364
UniProtKB accession
RefSeq Protein accession NP_417654.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: ispB
  • locus tag: b3187
  • aliases: ECK3176, JW3154, cel, yhbD
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB:
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG10017
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Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
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Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction octaprenyl diphosphate synthase
Source References Functional description
[PMID: 8037730]
Octaprenyl-diphosphate synthase (source organism: Escherichia coli (strain K12).; strain specific ID: K12 / JM109 / ATCC 53323)
Functional Status greenGreen (experimental evidence, uncurated)
EC number 2.5.1.- (See reaction via BRENDA)) (Source: NCBI RefSeq)
GO terms
  • MF: GO:0000287 : magnesium ion binding : IEA
  • MF: GO:0046872 : metal ion binding : IEA
  • BP: GO:0016094 : polyprenol biosynthetic process : IMP
  • BP: GO:0008299 : isoprenoid biosynthetic process : IEA
  • MF: GO:0005515 : protein binding : IPI
  • MF: GO:0004659 : prenyltransferase activity : IDA
  • MF: GO:0016740 : transferase activity : IEA
Domain Structure from CDD
  • Trans_IPPS_HT: Trans-Isoprenyl Diphosphate Synthases, head-to-tail. These trans-Isoprenyl Diphosphate Synthases (Trans_IPPS) catalyze h.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
cel , EcolC_0513 , ECP_3274 , ECs4066 , ECSE_3471 , Ent638_3623 , ESA_03580 , ispB (43) , NT01EI_0448 , SPAB_04122 , Spro_0472 , SSPA2960
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 phylogenetic profile
nthDNA glycosylase and apyrimidinic (AP) lyase (endon
ispUundecaprenyl pyrophosphate synthase
Metabolic Annotation from Palsson Lab

Metabolic Reaction: OCTDPS

Octaprenyl pyrophosphate synthase
frdp[c] + 5 ipdp[c] -> octdp[c] + 5 ppi[c]
Cofactor and Prosthetic Group Biosynthesis

Source: Ported to COMBREX by Segre's Lab
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(candidate essential)