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Gene prfB from Escherichia coli str. K-12 substr. MG1655: peptide chain release factor RF-2
Member of NCBI Protein Clusters PRK00578(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 947369
UniProtKB accession
RefSeq Protein accession NP_417367.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: prfB
  • locus tag: b2891
  • aliases: ECK2886, JW5847, supK
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 1GQE 1MI6 1ML5
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG10762
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Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction peptide chain release factor 2
Source References Functional description
[PMID: 3889910]
[PMID: 11118225]
Peptide chain release factor 2 (source organism: Escherichia coli (strain K12).; strain specific ID: )
Functional Status greenGreen (experimental evidence, uncurated)
GO terms
  • CC: GO:0005737 : cytoplasm : IEA
  • MF: GO:0003747 : translation release factor activity : IEA
  • BP: GO:0006412 : translation : IEA
  • MF: GO:0016149 : translation release factor activity, codon specific : IEA
  • BP: GO:0006415 : translational termination : IEA
Domain Structure from CDD
  • RF-1: RF-1 domain. peptidyl-tRNA hydrolase domain protein; Provisional
  • prfB: peptide chain release factor 2; Validated

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
ECP_2884 , ECSE_3154 , prfB (35)
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 gene neighbourhoods
phylogenetic profile
lysSlysine tRNA synthetase, constitutive
priAPrimosome factor n' (replication factor Y) 
ftsWintegral membrane protein involved in stabilizing  
murCUDP-N-acetylmuramate:L-alanine ligase 
clpPproteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP 
miaAdelta(2)-isopentenylpyrophosphate tRNA-adenosine t 
mrdBcell wall shape-determining protein 
ftsItranspeptidase involved in septal peptidoglycan sy 
murBUDP-N-acetylenolpyruvoylglucosamine reductase, FAD 
pnppolynucleotide phosphorylase/polyadenylase 
fabB3-oxoacyl-[acyl-carrier-protein] synthase I 
clpXATPase and specificity subunit of ClpX-ClpP ATP-de 
ccafused tRNA nucleotidyl transferase/2'3'-cyclic pho 
mrdAtranspeptidase involved in peptidoglycan synthesis 
rpsA30S ribosomal subunit protein S1 
fabF3-oxoacyl-[acyl-carrier-protein] synthase II 
polAfused DNA polymerase I 5'->3' polymerase/3'->5' ex 
murAUDP-N-acetylglucosamine 1-carboxyvinyltransferase 
pcnBpoly(A) polymerase I 
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(not essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(not essential)