Combrex Enter Keyword, gene symbol, or identifier (more options; Help )
Enter species name
Gene functional status (select)
Phenotype association (select)[View list of phenotypes]
Check to restrict search to:
Genes in Category A-C and Emerging/Re-Emerging pathogens
Genes in curated Protein Clusters
Genes with predictions submitted to COMBREX
Genes with solved structures in PDB
Genes with Pfam domains shared with human proteins
Genes with products purified by PSI
Genes cloned by PSI
close options


Gene fmt from Escherichia coli str. K-12 substr. MG1655: 10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase
Member of NCBI Protein Clusters PRK00005(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 947779
UniProtKB accession
RefSeq Protein accession NP_417746.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: fmt
  • locus tag: b3288
  • aliases: ECK3274, JW3249, yhdD
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 1FMT 2FMT
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG11268
Initiate the grant application process for experimentally validating this gene (Important notice about COMBREX grants.)
Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction methionyl-tRNA formyltransferase
Source References Functional description
[PMID: 1624424]
[PMID: 8887566]
[PMID: 9843487]
Methionyl-tRNA formyltransferase (source organism: Escherichia coli (strain K12).; strain specific ID: K12 / K37)
Functional Status greenGreen (experimental evidence, uncurated)
EC number
GO terms
  • BP: GO:0009058 : biosynthetic process : IEA
  • MF: GO:0004479 : methionyl-tRNA formyltransferase activity : IEA
  • MF: GO:0003824 : catalytic activity : IEA
  • MF: GO:0008168 : methyltransferase activity : IEA
  • BP: GO:0006412 : translation : IEA
  • BP: GO:0019988 : charged-tRNA amino acid modification : IDA
  • MF: GO:0004479 : methionyl-tRNA formyltransferase activity : IDA
  • MF: GO:0016740 : transferase activity : IEA
  • MF: GO:0016742 : hydroxymethyl-, formyl- and related transferase activity : IEA
Domain Structure from CDD
  • Formyl_trans_C: Formyl transferase, C-terminal domain.
  • Formyl_trans_N: Formyl transferase. phosphoribosylglycinamide formyltransferase; Reviewed
  • fmt: methionyl-tRNA formyltransferase; Reviewed

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
ESA_00038 , fmt (49)
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 domain fusion
gene neighbourhoods
phylogenetic profile
fadDacyl-CoA synthetase (long-chain-fatty-acid--CoA li
defpeptide deformylase 
rplQ50S ribosomal subunit protein L17  
rplI50S ribosomal subunit protein L9  
prfApeptide chain release factor RF-1  
rluApseudouridine synthase for 23S rRNA (position 746)  
rpsP30S ribosomal subunit protein S16  
rpmI50S ribosomal subunit protein L35  
pthpeptidyl-tRNA hydrolase  
tufBprotein chain elongation factor EF-Tu (duplicate o  
aspSaspartyl-tRNA synthetase  
frrribosome recycling factor  
truCtRNA U65 pseudouridine synthase  
rpsR30S ribosomal subunit protein S18  
rluC23S rRNA pseudouridine synthase; specific to U955,  
yidCmembrane protein insertase  
obgEGTPase involved in cell partioning and DNA repair  
tufAprotein chain elongation factor EF-Tu (duplicate o  
rplS50S ribosomal subunit protein L19  
rplL50S ribosomal subunit protein L7/L12  
rpsF30S ribosomal subunit protein S6  
mnmAtRNA (5-methylaminomethyl-2-thiouridylate)-methylt  
rpmG50S ribosomal subunit protein L33  
ftsHprotease, ATP-dependent zinc-metallo  
ssbsingle-stranded DNA-binding protein  
rncRNase III  
rpmA50S ribosomal subunit protein L27  
tsfprotein chain elongation factor EF-Ts  
lepAback-translocating Elongation Factor EF4, GTPase  
acpPacyl carrier protein (ACP)  
rluD23S rRNA pseudouridine synthase; specific to U1911  
prrgamma-aminobutyraldehyde dehydrogenase
gabDsuccinate-semialdehyde dehydrogenase I, NADP-depen
aldAaldehyde dehydrogenase A, NAD-linked
putAfused DNA-binding transcriptional regulator/prolin
entFenterobactin synthase multienzyme complex componen
aasfused 2-acylglycerophospho-ethanolamine acyl trans
entE2,3-dihydroxybenzoate-AMP ligase component of ente
sadsuccinate semialdehyde dehydrogenase, NAD(P)+-depe
betBbetaine aldehyde dehydrogenase, NAD-dependent
astDsuccinylglutamic semialdehyde dehydrogenase
puuCgamma-Glu-gamma-aminobutyraldehyde dehydrogenase,
aldBaldehyde dehydrogenase B
arnAfused UDP-L-Ara4N formyltransferase/UDP-GlcA C-4'-
feaBphenylacetaldehyde dehydrogenase
fadKshort chain acyl-CoA synthetase, anaerobic
caiCprobable crotonobetaine/carnitine-CoA ligase
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(candidate essential)