Combrex Enter Keyword, gene symbol, or identifier (more options; Help )
Enter species name
Gene functional status (select)
Phenotype association (select)[View list of phenotypes]
Check to restrict search to:
Genes in Category A-C and Emerging/Re-Emerging pathogens
Genes in curated Protein Clusters
Genes with predictions submitted to COMBREX
P
Genes with solved structures in PDB
S
Genes with Pfam domains shared with human proteins
H
Genes with products purified by PSI
U
Genes cloned by PSI
C
close options

 

Gene rluF from Escherichia coli str. K-12 substr. MG1655: 23S rRNA U2604 pseudouridine synthase

Hide | Top

Summary
Member of NCBI Protein Clusters PRK10475(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 948519
UniProtKB accession
RefSeq Protein accession NP_418446.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: rluF
  • locus tag: b4022
  • aliases: ECK4014, JW3982, yjbC
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 2GML 3DH3
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG11921

Hide | Top

Actions You Can Take
Initiate the grant application process for experimentally validating this gene (Important notice about COMBREX grants.)
Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
Post a comment about this gene to appear on this page (info).

Hide | Top

Function Predictions
Source Predicted function(s)
NCBI Protein Cluster Prediction 23S rRNA pseudouridine synthase F

Hide | Top

Experimental Validation
Source References Functional description
GSDB Under Review ribosomal large subunit pseudouridine synthase F (source organism: Escherichia coli K12; strain specific ID: NCBI_TaxID=83333)

Hide | Top

Supporting Functional Information
Functional Status greenGreen (experimental evidence, uncurated)
EC number 4.2.1.70 (See reaction via BRENDA)) (Source: NCBI RefSeq)
GO terms
  • MF: GO:0016866 : intramolecular transferase activity : IEA
  • BP: GO:0009451 : RNA modification : IEA
  • MF: GO:0016853 : isomerase activity : IEA
  • BP: GO:0000455 : enzyme-directed rRNA pseudouridine synthesis : IMP
  • MF: GO:0009982 : pseudouridine synthase activity : IMP
  • MF: GO:0003723 : RNA binding : IEA
  • MF: GO:0009982 : pseudouridine synthase activity : IEA
  • BP: GO:0001522 : pseudouridine synthesis : IEA
  • BP: GO:0006364 : rRNA processing : IEA
Domain Structure from CDD
  • PseudoU_synth_RluF: PseudoU_synth_RluF_like: Pseudouridine synthase, Escherichia coli RluF like. This group is comprised of bacterial protei.... (More)
  • PRK10475: 23S rRNA pseudouridine synthase F; Provisional
  • S4: S4/Hsp/ tRNA synthetase RNA-binding domain; The domain surface is populated by conserved, charged residues that define a.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
CKO_03898 , EcolC_4008 , ECP_4240 , ECs5005 , ECSE_4307 , Ent638_0226 , rluF (9) , SARI_03464 , SeAg_B4444 , SeD_A4604 , SeHA_C4528 , SeSA_A4405 , SNSL254_A4530 , SPAB_05187 , SSPA3740 , yjbC (27) , YPK_3069 , YPTB1058 , YPTS_1109

Hide | Top

Functionally Linked Genes
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see www.java.com
 phylogenetic profile
mrdBcell wall shape-determining protein
 
qseBquorum sensing DNA-binding response regulator in t
 
rstAmulticopy supressor of yjeE, yeaZ or ygjD deletion
 
coaDpantetheine-phosphate adenylyltransferase
 
murBUDP-N-acetylenolpyruvoylglucosamine reductase, FAD
 
polAfused DNA polymerase I 5'->3' polymerase/3'->5' ex
 
rpsA30S ribosomal subunit protein S1
 
kdpEDNA-binding response regulator in two-component re
 
baeRDNA-binding response regulator in two-component re
 
ftsWintegral membrane protein involved in stabilizing
 
mplUDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-mes
 
ddlAD-alanine-D-alanine ligase A
 
yggWpredicted oxidoreductase, HemN family
 
clpXATPase and specificity subunit of ClpX-ClpP ATP-de
 
mrdAtranspeptidase involved in peptidoglycan synthesis
 
hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H de
 
murCUDP-N-acetylmuramate:L-alanine ligase
 
clpPproteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP
 
phoBDNA-binding response regulator in two-component re
 
ompRDNA-binding response regulator in two-component re
 
basRDNA-binding response regulator in two-component re
 
phoPDNA-binding response regulator in two-component re
 
mfdtranscription-repair coupling factor
 
recGATP-dependent DNA helicase
 
torRDNA-binding response regulator in two-component re
 
murAUDP-N-acetylglucosamine 1-carboxyvinyltransferase
 
creBDNA-binding response regulator in two-component re
 
ftsItranspeptidase involved in septal peptidoglycan sy
 
priAPrimosome factor n' (replication factor Y)
 
ddlBD-alanine:D-alanine ligase
 
arcADNA-binding response regulator in two-component re
 
rsmD16S rRNA m(2)G966 methyltransferase, SAM-dependent
 
cpxRDNA-binding response regulator in two-component re
 
cusRDNA-binding response regulator in two-component re
 
yedWpredicted DNA-binding response regulator in two-co
 
rimM16S rRNA processing protein