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Gene prfA from Escherichia coli str. K-12 substr. MG1655: peptide chain release factor RF-1
Member of NCBI Protein Clusters PRK00591(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 949002
UniProtKB accession
RefSeq Protein accession NP_415729.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: prfA
  • locus tag: b1211
  • aliases: ECK1199, JW1202, sueB, uar, ups?
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 2B3T
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG10761
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Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction peptide chain release factor 1
Source References Functional description
[PMID: 11118225]
Peptide chain release factor 1 (source organism: Escherichia coli (strain K12).; strain specific ID: )
Functional Status greenGreen (experimental evidence, uncurated)
GO terms
  • CC: GO:0005737 : cytoplasm : IEA
  • MF: GO:0003747 : translation release factor activity : IEA
  • BP: GO:0006412 : translation : IEA
  • MF: GO:0016149 : translation release factor activity, codon specific : IEA
  • BP: GO:0006415 : translational termination : IEA
Domain Structure from CDD
  • RF-1: RF-1 domain. peptidyl-tRNA hydrolase domain protein; Provisional
  • prfA: peptide chain release factor 1; Validated

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
NT01EI_1564 , prfA (68)
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 gene neighbourhoods
phylogenetic profile
prmCN5-glutamine methyltransferase, modifies release f
hemAglutamyl tRNA reductase
yidCmembrane protein insertase 
arnAfused UDP-L-Ara4N formyltransferase/UDP-GlcA C-4'- 
tufAprotein chain elongation factor EF-Tu (duplicate o 
rplS50S ribosomal subunit protein L19 
fmt10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N 
rpmG50S ribosomal subunit protein L33 
tsfprotein chain elongation factor EF-Ts 
rplL50S ribosomal subunit protein L7/L12 
lepAback-translocating Elongation Factor EF4, GTPase 
rpmA50S ribosomal subunit protein L27 
rncRNase III 
rplI50S ribosomal subunit protein L9 
obgEGTPase involved in cell partioning and DNA repair 
rpsR30S ribosomal subunit protein S18 
ssbsingle-stranded DNA-binding protein 
frrribosome recycling factor 
rplQ50S ribosomal subunit protein L17 
truCtRNA U65 pseudouridine synthase 
rpmI50S ribosomal subunit protein L35 
acpPacyl carrier protein (ACP) 
rluD23S rRNA pseudouridine synthase; specific to U1911 
rluC23S rRNA pseudouridine synthase; specific to U955, 
rpsF30S ribosomal subunit protein S6 
ftsHprotease, ATP-dependent zinc-metallo 
mnmAtRNA (5-methylaminomethyl-2-thiouridylate)-methylt 
rluApseudouridine synthase for 23S rRNA (position 746) 
rpsP30S ribosomal subunit protein S16 
pthpeptidyl-tRNA hydrolase 
tufBprotein chain elongation factor EF-Tu (duplicate o 
aspSaspartyl-tRNA synthetase 
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(not essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(candidate essential)