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Gene mfd from Escherichia coli str. K-12 substr. MG1655: transcription-repair coupling factor
Member of NCBI Protein Clusters PRK10689(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 945681
UniProtKB accession
RefSeq Protein accession NP_415632.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: mfd
  • locus tag: b1114
  • aliases: ECK1100, JW1100
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 2B2N 2EYQ 3HJH
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG11619
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Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
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Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction transcription-repair coupling factor
Source References Functional description
GSDB Under Review transcription-repair-coupling factor (source organism: Escherichia coli K12; strain specific ID: NCBI_TaxID=83333)
Functional Status greenGreen (experimental evidence, uncurated)
GO terms
  • MF: GO:0005524 : ATP binding : IEA
  • MF: GO:0008026 : ATP-dependent helicase activity : IEA
  • BP: GO:0006281 : DNA repair : IEA
  • MF: GO:0003676 : nucleic acid binding : IEA
  • MF: GO:0005515 : protein binding : IPI
  • MF: GO:0003677 : DNA binding : IEA
  • MF: GO:0003700 : transcription factor activity : IEA
  • MF: GO:0000166 : nucleotide binding : IEA
  • BP: GO:0006974 : response to DNA damage stimulus : IEA
  • BP: GO:0006355 : regulation of transcription, DNA-dependent : IEA
  • MF: GO:0004386 : helicase activity : IEA
  • MF: GO:0016787 : hydrolase activity : IEA
  • MF: GO:0003684 : damaged DNA binding : IEA
Domain Structure from CDD
  • DEXDc: DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domai.... (More)
  • PRK10689: transcription-repair coupling factor; Provisional
  • CarD_TRCF: CarD-like/TRCF domain. Transcriptional regulators, similar to M. xanthus CarD [Transcription]
  • HELICc: Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A.... (More)
  • TRCF: TRCF domain.

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
CKO_01937 , EcolC_2487 , ECP_1106 , ECs1492 , ECSE_1179 , Ent638_1629 , ESA_02230 , mfd (49) , SARI_01775 , SPAB_02303 , SSPA1519 , YPA_1900 , YPDSF_1824 , YPK_1706 , YPN_2007 , YPTS_2527
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see www.java.com
 phylogenetic profile
basRDNA-binding response regulator in two-component re
 
cusRDNA-binding response regulator in two-component re
 
ftsItranspeptidase involved in septal peptidoglycan sy
 
kdpEDNA-binding response regulator in two-component re
 
mplUDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-mes
 
mrdAtranspeptidase involved in peptidoglycan synthesis
 
hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H de
 
ddlBD-alanine:D-alanine ligase
 
phoBDNA-binding response regulator in two-component re
 
ompRDNA-binding response regulator in two-component re
 
typAGTP-binding protein
 
murDUDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
 
rluE23S rRNA U2457 pseudouridine synthase
 
pnppolynucleotide phosphorylase/polyadenylase
 
recGATP-dependent DNA helicase
 
torRDNA-binding response regulator in two-component re
 
murEUDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-dia
 
rluF23S rRNA U2604 pseudouridine synthase
 
rsmD16S rRNA m(2)G966 methyltransferase, SAM-dependent
 
yedWpredicted DNA-binding response regulator in two-co
 
murBUDP-N-acetylenolpyruvoylglucosamine reductase, FAD
 
priAPrimosome factor n' (replication factor Y)
 
qseBquorum sensing DNA-binding response regulator in t
 
polAfused DNA polymerase I 5'->3' polymerase/3'->5' ex
 
murCUDP-N-acetylmuramate:L-alanine ligase
 
clpPproteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP
 
rimM16S rRNA processing protein
 
phoPDNA-binding response regulator in two-component re
 
arcADNA-binding response regulator in two-component re
 
ddlAD-alanine-D-alanine ligase A
 
creBDNA-binding response regulator in two-component re
 
clpXATPase and specificity subunit of ClpX-ClpP ATP-de
 
cpxRDNA-binding response regulator in two-component re
 
miaAdelta(2)-isopentenylpyrophosphate tRNA-adenosine t
 
mrdBcell wall shape-determining protein
 
rstAmulticopy supressor of yjeE, yeaZ or ygjD deletion
 
murAUDP-N-acetylglucosamine 1-carboxyvinyltransferase
 
rsuA16S rRNA U516 pseudouridine synthase
 
xseAexonuclease VII, large subunit
 
yggWpredicted oxidoreductase, HemN family
 
baeRDNA-binding response regulator in two-component re
 
ftsWintegral membrane protein involved in stabilizing
 
rluB23S rRNA pseudouridylate synthase
 
Phenotype Description Expression
Class
Reference(s)
Ampicillin sensitivity Knockout confers increased sensitivity to ampicillin Knock out
PMID: 20065048
Cephradine sensitivity Knockout confers increased sensitivity to cephradine Knock out
PMID: 20065048
Ciprofloxacin sensitivity Knockout confers increased sensitivity to ciprofloxacin Knock out
PMID: 20065048
Gentamicin sensitivity Knockout confers increased sensitivity to gentamicin Knock out
PMID: 20065048
Metronidazole sensitivity Knockout confers increased sensitivity to metronidazole Knock out
PMID: 20065048
Neomycin sensitivity Knockout confers increased sensitivity to neomycin Knock out
PMID: 20065048
Nitrofurantoin sensitivity Knockout confers increased sensitivity to nitrofurantoin Knock out
PMID: 20065048
Streptomycin sensitivity Knockout confers increased sensitivity to streptomycin Knock out
PMID: 20065048
Tobramycin sensitivity Knockout confers increased sensitivity to tobramycin Knock out
PMID: 20065048