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Gene murC from Escherichia coli str. K-12 substr. MG1655: UDP-N-acetylmuramate:L-alanine ligase
Member of NCBI Protein Clusters PRK00421(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 946153
UniProtKB accession
RefSeq Protein accession NP_414633.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: murC
  • locus tag: b0091
  • aliases: ECK0092, JW0089
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 1CC9 2F00
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG10619
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Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction UDP-N-acetylmuramate--L-alanine ligase
Source References Functional description
[PMID: 7601127]
UDP-N-acetylmuramate--L-alanine ligase (source organism: Escherichia coli (strain K12).; strain specific ID: )
Functional Status greenGreen (experimental evidence, uncurated)
EC number
GO terms
  • MF: GO:0008763 : UDP-N-acetylmuramate-L-alanine ligase activity : IDA
  • MF: GO:0016874 : ligase activity : IEA
  • BP: GO:0007049 : cell cycle : IEA
  • MF: GO:0000287 : magnesium ion binding : IDA
  • BP: GO:0009252 : peptidoglycan biosynthetic process : IEA
  • BP: GO:0008360 : regulation of cell shape : IEA
  • BP: GO:0051301 : cell division : IEA
  • MF: GO:0000166 : nucleotide binding : IEA
  • CC: GO:0005737 : cytoplasm : IEA
  • BP: GO:0007047 : cellular cell wall organization : IEA
  • BP: GO:0008152 : metabolic process : IEA
  • MF: GO:0008763 : UDP-N-acetylmuramate-L-alanine ligase activity : IEA
  • BP: GO:0009273 : peptidoglycan-based cell wall biogenesis : IEA
  • MF: GO:0005488 : binding : IEA
  • MF: GO:0005515 : protein binding : IPI
  • MF: GO:0005524 : ATP binding : IDA
  • BP: GO:0009058 : biosynthetic process : IEA
  • MF: GO:0003824 : catalytic activity : IEA
  • MF: GO:0005524 : ATP binding : IEA
Domain Structure from CDD
  • Mur_ligase_C: Mur ligase family, glutamate ligase domain. This family contains a number of related ligase enzymes which have EC number.... (More)
  • murC: UDP-N-acetylmuramate--L-alanine ligase; Provisional
  • Mur_ligase_M: Mur ligase middle domain.
  • Mur_ligase: Mur ligase family, catalytic domain. This family contains a number of related ligase enzymes which have EC numbers 6.3.2.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
murC (69) , NT01EI_0737
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 phylogenetic profile
gene neighbourhoods
domain fusion
rluE23S rRNA U2457 pseudouridine synthase
murGN-acetylglucosaminyl transferase
ddlBD-alanine:D-alanine ligase
rstAmulticopy supressor of yjeE, yeaZ or ygjD deletion
mrdAtranspeptidase involved in peptidoglycan synthesis
hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H de
cusRDNA-binding response regulator in two-component re
prfBpeptide chain release factor RF-2
baeRDNA-binding response regulator in two-component re
ftsWintegral membrane protein involved in stabilizing
phoBDNA-binding response regulator in two-component re
ompRDNA-binding response regulator in two-component re
ccafused tRNA nucleotidyl transferase/2'3'-cyclic pho
murDUDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
rimM16S rRNA processing protein
qseBquorum sensing DNA-binding response regulator in t
polAfused DNA polymerase I 5'->3' polymerase/3'->5' ex
pnppolynucleotide phosphorylase/polyadenylase
recGATP-dependent DNA helicase
glmUfused N-acetyl glucosamine-1-phosphate uridyltrans
torRDNA-binding response regulator in two-component re
rluF23S rRNA U2604 pseudouridine synthase
rsmD16S rRNA m(2)G966 methyltransferase, SAM-dependent
yajCSecYEG protein translocase auxillary subunit
ddlAD-alanine-D-alanine ligase A
yggWpredicted oxidoreductase, HemN family
clpXATPase and specificity subunit of ClpX-ClpP ATP-de
yedWpredicted DNA-binding response regulator in two-co
pcnBpoly(A) polymerase I
nlpDnovel lipoprotein, function unknown
murBUDP-N-acetylenolpyruvoylglucosamine reductase, FAD
priAPrimosome factor n' (replication factor Y)
secGpreprotein translocase membrane subunit
yaeJconserved protein
clpPproteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP
radADNA repair protein
rluB23S rRNA pseudouridylate synthase
ygeRnovel lipoprotein, function unknown
basRDNA-binding response regulator in two-component re
phoPDNA-binding response regulator in two-component re
mfdtranscription-repair coupling factor
yebApredicted peptidase
murAUDP-N-acetylglucosamine 1-carboxyvinyltransferase
creBDNA-binding response regulator in two-component re
ftsItranspeptidase involved in septal peptidoglycan sy
rsuA16S rRNA U516 pseudouridine synthase
arcADNA-binding response regulator in two-component re
rpsA30S ribosomal subunit protein S1
kdpEDNA-binding response regulator in two-component re
cpxRDNA-binding response regulator in two-component re
miaAdelta(2)-isopentenylpyrophosphate tRNA-adenosine t
mrdBcell wall shape-determining protein
Metabolic Annotation from Palsson Lab

Metabolic Reaction: UAMAS

UDP-N-acetylmuramoyl-L-alanine synthetase
ala-DASH-L[c] + atp[c] + uamr[c] -> adp[c] + h[c] + pi[c] + uama[c]
Cell Envelope Biosynthesis

Source: Ported to COMBREX by Segre's Lab
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(candidate essential)