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Gene polA from Escherichia coli str. K-12 substr. MG1655: fused DNA polymerase I 5'->3' polymerase/3'->5' exonuclease/5'->3' exonuclease
Member of NCBI Protein Clusters PRK05755(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 948356
UniProtKB accession
RefSeq Protein accession NP_418300.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: polA
  • locus tag: b3863
  • aliases: ECK3855, JW3835, resA
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
This gene is already gold standard. If you believe there is an additional, validatable function not described here, please contact us.
Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
This gene is already gold standard. If you believe there is an additional, validatable function not described here, please contact us.
Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction DNA polymerase I
Source References Functional description
[PMID: 13363894]
[PMID: 13563462]
[PMID: 4890763]
[PMID: 14114849]
DNA polymerase I (fused 5'- (source organism: Escherichia coli (strain K12).; strain specific ID: ) / Curator Brian Anton (record returned: Jun 29 2011)
Functional Status greenGold (experimental evidence, curated)
EC number
GO terms
  • MF: GO:0004527 : exonuclease activity : IEA
  • CC: GO:0005622 : intracellular : IEA
  • BP: GO:0006281 : DNA repair : IEA
  • CC: GO:0005737 : cytoplasm : IDA
  • MF: GO:0003676 : nucleic acid binding : IEA
  • MF: GO:0004518 : nuclease activity : IEA
  • MF: GO:0008408 : 3'-5' exonuclease activity : IEA
  • MF: GO:0003887 : DNA-directed DNA polymerase activity : IEA
  • BP: GO:0006974 : response to DNA damage stimulus : IEA
  • MF: GO:0016787 : hydrolase activity : IEA
  • BP: GO:0006139 : nucleobase, nucleoside, nucleotide and nucleic acid metabolic process : IEA
  • MF: GO:0008409 : 5'-3' exonuclease activity : IEA
  • MF: GO:0005515 : protein binding : IPI
  • MF: GO:0003677 : DNA binding : IEA
  • MF: GO:0016740 : transferase activity : IEA
  • MF: GO:0016779 : nucleotidyltransferase activity : IEA
  • BP: GO:0006260 : DNA replication : IEA
  • MF: GO:0003824 : catalytic activity : IEA
Domain Structure from CDD
  • DNA_pol_A: DNA polymerase family A, 5'-3' polymerase domain. Family A polymerase (polymerase I) functions primarily to fill DNA gap.... (More)
  • 53EXOc: 5'-3' exonuclease; T5 type 5'-3' exonuclease domains may co-occur with DNA polymerase I (Pol I) domains, or be part of P.... (More)
  • DNA_polA_I_Ecoli_like_exo: The 3'-5' exonuclease domain of DNA polymerases has a fundamental role in reducing polymerase errors and is involved in .... (More)
  • PRK05755: DNA polymerase I; Provisional

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
CKO_03151 , EcolC_4152 , ECP_4074 , ECs4786 , ECSE_4145 , Ent638_4102 , ESA_04040 , polA (35) , SARI_03661 , SeAg_B4231 , SeD_A4388 , SeHA_C4327 , SNSL254_A4281 , SPAB_04960 , SSPA3571
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 phylogenetic profile
pnppolynucleotide phosphorylase/polyadenylase
recGATP-dependent DNA helicase
torRDNA-binding response regulator in two-component re
rluF23S rRNA U2604 pseudouridine synthase
accCacetyl-CoA carboxylase, biotin carboxylase subunit
ddlAD-alanine-D-alanine ligase A
yedWpredicted DNA-binding response regulator in two-co
murBUDP-N-acetylenolpyruvoylglucosamine reductase, FAD
priAPrimosome factor n' (replication factor Y)
yaeJconserved protein
murCUDP-N-acetylmuramate:L-alanine ligase
clpPproteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP
phoPDNA-binding response regulator in two-component re
arcADNA-binding response regulator in two-component re
creBDNA-binding response regulator in two-component re
cpxRDNA-binding response regulator in two-component re
radADNA repair protein
murAUDP-N-acetylglucosamine 1-carboxyvinyltransferase
rsuA16S rRNA U516 pseudouridine synthase
clpXATPase and specificity subunit of ClpX-ClpP ATP-de
ddlBD-alanine:D-alanine ligase
cusRDNA-binding response regulator in two-component re
ftsItranspeptidase involved in septal peptidoglycan sy
pcnBpoly(A) polymerase I
mfdtranscription-repair coupling factor
rluE23S rRNA U2457 pseudouridine synthase
qseBquorum sensing DNA-binding response regulator in t
rpsA30S ribosomal subunit protein S1
kdpEDNA-binding response regulator in two-component re
miaAdelta(2)-isopentenylpyrophosphate tRNA-adenosine t
mrdBcell wall shape-determining protein
rstAmulticopy supressor of yjeE, yeaZ or ygjD deletion
rluB23S rRNA pseudouridylate synthase
mrdAtranspeptidase involved in peptidoglycan synthesis
prfBpeptide chain release factor RF-2
ccafused tRNA nucleotidyl transferase/2'3'-cyclic pho
baeRDNA-binding response regulator in two-component re
ftsWintegral membrane protein involved in stabilizing
basRDNA-binding response regulator in two-component re
phoBDNA-binding response regulator in two-component re
ompRDNA-binding response regulator in two-component re
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(not essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(not essential)