Combrex Enter Keyword, gene symbol, or identifier (more options; Help )
Enter species name
Gene functional status (select)
Phenotype association (select)[View list of phenotypes]
Check to restrict search to:
Genes in Category A-C and Emerging/Re-Emerging pathogens
Genes in curated Protein Clusters
Genes with predictions submitted to COMBREX
Genes with solved structures in PDB
Genes with Pfam domains shared with human proteins
Genes with products purified by PSI
Genes cloned by PSI
close options


Gene hemN from Escherichia coli str. K-12 substr. MG1655: coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen-independent
Member of NCBI Protein Clusters PRK09249(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 948362
UniProtKB accession
RefSeq Protein accession NP_418303.2 (PROVISIONAL)
Gene Symbol(s)
  • symbol: hemN
  • locus tag: b3867
  • aliases: ECK3860, JW3838, yihJ
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
  • S
    Structure(s) available in PDB: 1OLT
  • C
    Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.)
  • U
    Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium).
  • Protein described in EcoCyc: EG11836
Initiate the grant application process for experimentally validating this gene (Important notice about COMBREX grants.)
Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
Post a comment about this gene to appear on this page (info).
Source Predicted function(s)
NCBI Protein Cluster Prediction coproporphyrinogen III oxidase
Source References Functional description
[PMID: 12114526]
[PMID: 15967800]
[PMID: 14633981]
Oxygen-independent coproporphyrinogen-III oxidase (source organism: Escherichia coli (strain K12).; strain specific ID: )
Functional Status greenGreen (experimental evidence, uncurated)
EC number
GO terms
  • BP: GO:0055114 : oxidation reduction : IEA
  • MF: GO:0046872 : metal ion binding : IEA
  • MF: GO:0003824 : catalytic activity : IEA
  • MF: GO:0051539 : 4 iron, 4 sulfur cluster binding : IDA
  • MF: GO:0051989 : coproporphyrinogen dehydrogenase activity : IDA
  • MF: GO:0051536 : iron-sulfur cluster binding : IEA
  • MF: GO:0004109 : coproporphyrinogen oxidase activity : IEA
  • MF: GO:0005506 : iron ion binding : IEA
  • MF: GO:0051989 : coproporphyrinogen dehydrogenase activity : IEA
  • CC: GO:0005737 : cytoplasm : IEA
  • BP: GO:0019353 : protoporphyrinogen IX biosynthetic process from glutamate : IGI
  • BP: GO:0006779 : porphyrin biosynthetic process : IEA
  • MF: GO:0051539 : 4 iron, 4 sulfur cluster binding : IEA
  • MF: GO:0016491 : oxidoreductase activity : IEA
Domain Structure from CDD
  • PRK09249: coproporphyrinogen III oxidase; Provisional
  • HemN_C: HemN C-terminal region. Members of this family are all oxygen-independent coproporphyrinogen-III oxidases (HemN). This e.... (More)
  • Radical_SAM: Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S cluster and S-adenosylmethionine.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
CKO_03145 , EcolC_4149 , ECP_4077 , ECs4789 , ECSE_4150 , Ent638_4099 , ESA_04045 , hemN (42) , SARI_03656 , SPAB_04966 , Spro_4884 , SSPA3575
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 phylogenetic profile
secGpreprotein translocase membrane subunit
murCUDP-N-acetylmuramate:L-alanine ligase
clpPproteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP
radADNA repair protein
rluB23S rRNA pseudouridylate synthase
mfdtranscription-repair coupling factor
rluF23S rRNA U2604 pseudouridine synthase
murAUDP-N-acetylglucosamine 1-carboxyvinyltransferase
ftsItranspeptidase involved in septal peptidoglycan sy
rsuA16S rRNA U516 pseudouridine synthase
priAPrimosome factor n' (replication factor Y)
mrdBcell wall shape-determining protein
mrdAtranspeptidase involved in peptidoglycan synthesis
rluE23S rRNA U2457 pseudouridine synthase
ftsWintegral membrane protein involved in stabilizing
murBUDP-N-acetylenolpyruvoylglucosamine reductase, FAD
clpXATPase and specificity subunit of ClpX-ClpP ATP-de