COMBREX: priA in Escherichia coli str. K-12 substr. MG1655

Summary

Member of NCBI Protein Clusters	PRK05580(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID	948426
UniProtKB accession	P17888
RefSeq Protein accession	NP_418370.1 (PROVISIONAL)
Gene Symbol(s)	symbol: priA locus tag: b3935 aliases: ECK3927, JW3906, srgA
Organism	Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:	S Structure(s) available in PDB: 2D7E 2D7G 2D7H 2DWL 2DWM 2DWN C Gene cloned by a participant in the Protein Structure Initiative (PSI): TargetDB. (To obtain a clone, please contact the PSI:Biology-Materials Repository.) U Protein purified by a participant in the Protein Structure Initiative (PSI): TargetDB. (If you are interested in collaborating to obtain the protein, please contact the Northeast Structural Genomics consortium). Protein described in EcoCyc: EG10763

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	Contribute a predicted function for this gene (free text, GO terms, or EC number) (info). Be sure to check the list of current predicted functions in the section immediately below beforehand.
	Nominate this gene for the Gold Standard Gene Database (if you believe it has been experimentally validated) (info).
	Post a comment about this gene to appear on this page (info).

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Function Predictions

Source	Predicted function(s)
NCBI Protein Cluster Prediction	primosome assembly protein PriA

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Experimental Validation

Source	References	Functional description
GSDB	Under Review	primosomal protein N' (source organism: Escherichia coli K12; strain specific ID: NCBI_TaxID=83333)

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Supporting Functional Information

Functional Status	Green (experimental evidence, uncurated)
GO terms	BP: GO:0006260 : DNA replication : IEA MF: GO:0046872 : metal ion binding : IEA MF: GO:0005524 : ATP binding : IEA MF: GO:0008026 : ATP-dependent helicase activity : IEA BP: GO:0006269 : DNA replication, synthesis of RNA primer : IEA MF: GO:0003676 : nucleic acid binding : IEA MF: GO:0008270 : zinc ion binding : IEA MF: GO:0005515 : protein binding : IPI MF: GO:0003677 : DNA binding : IEA MF: GO:0000166 : nucleotide binding : IEA MF: GO:0004386 : helicase activity : IEA MF: GO:0016787 : hydrolase activity : IEA CC: GO:0005658 : alpha DNA polymerase:primase complex : IEA
Domain Structure from CDD	DEXDc: DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region..... (More) PRK05580: primosome assembly protein PriA; Validated DEXDc: N/A. This represents a conserved region within a number of RAD3-like DNA-binding helicases that are seemingly ubiquitous.... (More) See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam	DEAD H Helicase_C H See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details)	[Download ] CKO_03059 , EcolC_4083 , ECP_4144 , ECs4862 , ECSE_4224 , Ent638_4038 , ESA_03825 , priA (36) , SARI_03561 , SeAg_B4341 , SeD_A4495 , SeHA_C4427 , SeSA_A4311 , SNSL254_A4425 , SPAB_05073 , SSPA3665

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Functionally Linked Genes

The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see www.java.com


prfB	peptide chain release factor RF-2
baeR	DNA-binding response regulator in two-component re
ftsW	integral membrane protein involved in stabilizing
cca	fused tRNA nucleotidyl transferase/2'3'-cyclic pho
rimM	16S rRNA processing protein
qseB	quorum sensing DNA-binding response regulator in t
polA	fused DNA polymerase I 5'->3' polymerase/3'->5' ex
yajC	SecYEG protein translocase auxillary subunit
ddlA	D-alanine-D-alanine ligase A
rpsA	30S ribosomal subunit protein S1
kdpE	DNA-binding response regulator in two-component re
mpl	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-mes
yggW	predicted oxidoreductase, HemN family
clpX	ATPase and specificity subunit of ClpX-ClpP ATP-de
pcnB	poly(A) polymerase I
secG	preprotein translocase membrane subunit
mrdA	transpeptidase involved in peptidoglycan synthesis
hemN	coproporphyrinogen III oxidase, SAM and NAD(P)H de
murG	N-acetylglucosaminyl transferase
murC	UDP-N-acetylmuramate:L-alanine ligase
clpP	proteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP
radA	DNA repair protein
phoB	DNA-binding response regulator in two-component re
ompR	DNA-binding response regulator in two-component re
murD	UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
rluB	23S rRNA pseudouridylate synthase
ygeR	novel lipoprotein, function unknown
basR	DNA-binding response regulator in two-component re
yaeJ	conserved protein
phoP	DNA-binding response regulator in two-component re
mfd	transcription-repair coupling factor
yebA	predicted peptidase
pnp	polynucleotide phosphorylase/polyadenylase
recG	ATP-dependent DNA helicase
glmU	fused N-acetyl glucosamine-1-phosphate uridyltrans
torR	DNA-binding response regulator in two-component re
murE	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-dia
murA	UDP-N-acetylglucosamine 1-carboxyvinyltransferase
rluF	23S rRNA U2604 pseudouridine synthase
rsmD	16S rRNA m(2)G966 methyltransferase, SAM-dependent
creB	DNA-binding response regulator in two-component re
yedW	predicted DNA-binding response regulator in two-co
nlpD	novel lipoprotein, function unknown
ftsI	transpeptidase involved in septal peptidoglycan sy
murB	UDP-N-acetylenolpyruvoylglucosamine reductase, FAD
rsuA	16S rRNA U516 pseudouridine synthase
ddlB	D-alanine:D-alanine ligase
arcA	DNA-binding response regulator in two-component re
cpxR	DNA-binding response regulator in two-component re
rluE	23S rRNA U2457 pseudouridine synthase
miaA	delta(2)-isopentenylpyrophosphate tRNA-adenosine t
mrdB	cell wall shape-determining protein
rstA	multicopy supressor of yjeE, yeaZ or ygjD deletion
cusR	DNA-binding response regulator in two-component re

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Pathway Information

KEGG	eco03440: Homologous recombination

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Phenotype data

Phenotype	Description	Expression Class	Reference(s)
Candidate essential gene	Identified as essential gene in one or more studies (see references)	Knock out	PMID: 13129938 (candidate essential) PMID: 17700540 (candidate essential) PMID: 20029369 (not essential)

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