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Gene priA from Escherichia coli str. K-12 substr. MG1655: Primosome factor n' (replication factor Y)
Member of NCBI Protein Clusters PRK05580(See COMBREX Page ) (See NCBI page)
NCBI Entrez GeneID 948426
UniProtKB accession
RefSeq Protein accession NP_418370.1 (PROVISIONAL)
Gene Symbol(s)
  • symbol: priA
  • locus tag: b3935
  • aliases: ECK3927, JW3906, srgA
Organism Escherichia coli str. K-12 substr. MG1655 (NCBI TaxID: 511145)
Other Cross References:
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Source Predicted function(s)
NCBI Protein Cluster Prediction primosome assembly protein PriA
Source References Functional description
GSDB Under Review primosomal protein N' (source organism: Escherichia coli K12; strain specific ID: NCBI_TaxID=83333)
Functional Status greenGreen (experimental evidence, uncurated)
GO terms
  • BP: GO:0006260 : DNA replication : IEA
  • MF: GO:0046872 : metal ion binding : IEA
  • MF: GO:0005524 : ATP binding : IEA
  • MF: GO:0008026 : ATP-dependent helicase activity : IEA
  • BP: GO:0006269 : DNA replication, synthesis of RNA primer : IEA
  • MF: GO:0003676 : nucleic acid binding : IEA
  • MF: GO:0008270 : zinc ion binding : IEA
  • MF: GO:0005515 : protein binding : IPI
  • MF: GO:0003677 : DNA binding : IEA
  • MF: GO:0000166 : nucleotide binding : IEA
  • MF: GO:0004386 : helicase activity : IEA
  • MF: GO:0016787 : hydrolase activity : IEA
  • CC: GO:0005658 : alpha DNA polymerase:primase complex : IEA
Domain Structure from CDD
  • DEXDc: DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domai.... (More)
  • PRK05580: primosome assembly protein PriA; Validated
  • DEXDc: N/A. This represents a conserved region within a number of RAD3-like DNA-binding helicases that are seemingly ubiquitous.... (More)

See domain structure on NCBI Conserved Domain Database
Domain structure from Pfam
See domain structure on Pfam Database
Based on the function of this gene, COMBREX predictions were made to the following related genes (mouse over gene symbol for details) [Download ]
CKO_03059 , EcolC_4083 , ECP_4144 , ECs4862 , ECSE_4224 , Ent638_4038 , ESA_03825 , priA (36) , SARI_03561 , SeAg_B4341 , SeD_A4495 , SeHA_C4427 , SeSA_A4311 , SNSL254_A4425 , SPAB_05073 , SSPA3665
The table at right lists genes that may be "functionally linked to" (i.e., participate in a common biological process, or form a protein complex with) the subject gene of this page, as determined by two sequence-independent methods, including phylogenetic profiling and operon membership (determined by OperonDB). (Info.) These linkages may also be viewed graphically using the program VisANT, by clicking on the headers of the table. Note: VisANT requires Java. To install the latest version of Java, see
 phylogenetic profile
prfBpeptide chain release factor RF-2
baeRDNA-binding response regulator in two-component re
ftsWintegral membrane protein involved in stabilizing
ccafused tRNA nucleotidyl transferase/2'3'-cyclic pho
rimM16S rRNA processing protein
qseBquorum sensing DNA-binding response regulator in t
polAfused DNA polymerase I 5'->3' polymerase/3'->5' ex
yajCSecYEG protein translocase auxillary subunit
ddlAD-alanine-D-alanine ligase A
rpsA30S ribosomal subunit protein S1
kdpEDNA-binding response regulator in two-component re
yggWpredicted oxidoreductase, HemN family
clpXATPase and specificity subunit of ClpX-ClpP ATP-de
pcnBpoly(A) polymerase I
secGpreprotein translocase membrane subunit
mrdAtranspeptidase involved in peptidoglycan synthesis
hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H de
murGN-acetylglucosaminyl transferase
murCUDP-N-acetylmuramate:L-alanine ligase
clpPproteolytic subunit of ClpA-ClpP and ClpX-ClpP ATP
radADNA repair protein
phoBDNA-binding response regulator in two-component re
ompRDNA-binding response regulator in two-component re
murDUDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase
rluB23S rRNA pseudouridylate synthase
ygeRnovel lipoprotein, function unknown
basRDNA-binding response regulator in two-component re
yaeJconserved protein
phoPDNA-binding response regulator in two-component re
mfdtranscription-repair coupling factor
yebApredicted peptidase
pnppolynucleotide phosphorylase/polyadenylase
recGATP-dependent DNA helicase
glmUfused N-acetyl glucosamine-1-phosphate uridyltrans
torRDNA-binding response regulator in two-component re
murAUDP-N-acetylglucosamine 1-carboxyvinyltransferase
rluF23S rRNA U2604 pseudouridine synthase
rsmD16S rRNA m(2)G966 methyltransferase, SAM-dependent
creBDNA-binding response regulator in two-component re
yedWpredicted DNA-binding response regulator in two-co
nlpDnovel lipoprotein, function unknown
ftsItranspeptidase involved in septal peptidoglycan sy
murBUDP-N-acetylenolpyruvoylglucosamine reductase, FAD
rsuA16S rRNA U516 pseudouridine synthase
ddlBD-alanine:D-alanine ligase
arcADNA-binding response regulator in two-component re
cpxRDNA-binding response regulator in two-component re
rluE23S rRNA U2457 pseudouridine synthase
miaAdelta(2)-isopentenylpyrophosphate tRNA-adenosine t
mrdBcell wall shape-determining protein
rstAmulticopy supressor of yjeE, yeaZ or ygjD deletion
cusRDNA-binding response regulator in two-component re
Phenotype Description Expression
Candidate essential gene Identified as essential gene in one or more studies (see references) Knock out
PMID: 13129938
(candidate essential)
PMID: 17700540
(candidate essential)
PMID: 20029369
(not essential)